Antigenic analysis of plasminogen and plasmin.

By UBALDO RIFi , FELIX MILGROM AND SIDNEY SHULMAN M ANY METHODS have been used to purify human plasminogen, including selective denaturation,1 salting out with ammonium sulfate,2 zone electrophoresis,3 and various types of column chromatography,48 among others.9’#{176} The resultant preparations have still shown physical heterogeneity. Similar heterogeneity has been seen in the corresponding plasmin preparations. 12 We felt, therefore, that it would be of interest, to apply the sensitive methods of serologic and immunochemical study for the analysis and identification of the components of pbasminogen and plasmin preparations. These methods would also be applicable to the comparison of the plasminogen and